"The effect of eukaryotic inhibitors was evaluated on growth and chitinase production of the recombinant yeast. The transcriptional inhibitors (8-hydroxyquinoline and 8-azaguanine) inhibited the growth of YT 5 significantly (< 20%) but in the presence of CHI (100 脙聜脗碌g/ml), more than 75% was observed. The protein profile was comparatively reduced to 6% and 20% at 100 脙聜脗碌g/ml of 8-hydroxyquinoline and 250 脙聜脗碌g/ml of 8-azaguanine, respectively when supplemented in the culture medium. Even though relative growth and protein concentrations were reduced in the presence of transcriptional inhibitors, chitinase activity was higher by 70%. In the presence of CHI (50 脙聜脗碌g/ml) the chitinase established 10 脙聜脗卤 1.06% of higher activity than the control but the growth and total protein had not been altered much by CHI.
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Last date updated on April, 2026