Effect of Silver Sulfadiazine on Mature Mixed Bacterial Biofilms on Voice Prostheses
*Corresponding Author:Received Date: Feb 28, 2025 / Published Date: Mar 30, 2025
Citation: Tsung-Lin Y (2025) Effect of Silver Sulfadiazine on Mature Mixed Bacterial Biofilms on Voice Prostheses. Otolaryngol (Sunnyvale) 15: 627.DOI: 10.4172/2161-119X.1000627
Copyright: © 2025 Tsung-Lin Y. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Voice prostheses (VPs) are commonly used in patients who have undergone laryngectomy, serving as essential tools for speech rehabilitation. However, these prostheses are prone to colonization by pathogenic microorganisms, forming mature mixed bacterial biofilms that can lead to infection, reduced prosthetic function, and overall clinical complications. This study aims to evaluate the effect of silver sulfadiazine (SSD) on mature mixed bacterial biofilms on voice prostheses. Using an in vitro model, we analyzed the impact of SSD on biofilm viability, structure, and composition over a 7-day period. Bacterial strains commonly associated with voice prosthesis infections, including Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis, were cultured to form mixed biofilms on silicone voice prostheses. Silver sulfadiazine was applied at concentrations of 0.5%, 1%, and 2%. Biofilm disruption was assessed using confocal laser scanning microscopy (CLSM) and the XTT assay for metabolic activity. The results indicated that SSD significantly reduced biofilm viability, with the 1% SSD concentration showing the most substantial effect in disrupting biofilm formation. Moreover, SSD was found to affect both the structural integrity and the metabolic activity of the biofilm. This study provides valuable insights into the potential role of silver sulfadiazine as a therapeutic agent for managing bacterial colonization on voice prostheses, offering a promising alternative for improving prosthesis longevity and patient outcomes.
