中国P站

Abstract

Ethyl acetoacetate (EAA) is a versatile building block in the synthesis of pharmaceuticals, agrochemicals, and flavors. Traditionally synthesized via the Claisen condensation using sodium ethoxide in ethanol, the process suffers from poor atom economy, generation of hazardous waste, and significant energy consumption. In this work, we present an eco-friendly enzymatic method for synthesizing EAA using lipase-catalyzed transesterification of ethyl acetate with acetylacetone. The process was optimized via response surface methodology (RSM), analyzing temperature, pH, substrate ratio, and enzyme concentration. The optimized reaction achieved a 93% yield at ambient conditions using Candida antarctica lipase B (CALB) as a biocatalyst. This study highlights the feasibility of replacing traditional chemical synthesis with sustainable enzymatic alternatives in large-scale organic manufacturing.