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Abstract

Asthma, a complex genetic disease is characterized by airway hyper-responsiveness to a variety of stimuli and airway inflammation. Autophagy Related Gene 5 (ATG5) plays an important role in autophagy pathway and genetic polymorphisms in this gene have been shown to associate with asthma in different populations. The goal of this study was to look for association between single nucleotide polymorphism (rs17587319 C/G) of ATG5 and asthma in North Indian population. For this, a case-control study including 94 asthmatic patients and 50 healthy controls was conducted. The genotypes frequencies were determined using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCRRFLP). The genotype frequencies of rs17587319 in asthmatic patients were CC, 91.49% (n=86); CG, 8.51% (n=8) and GG, 0% (n=0) with allele frequencies of C, 0.96 and G, 0.04. The genotypes frequencies in control group were CC, 96% (n=48); CG, 4% (n=2) and GG, 0% (n=0) with allele frequencies of C, 0.98 (98%) and G 0.02 (2%). The genotype frequencies were in Hardy Weinberg equilibrium (P=0.8853) in control group. Genotyping of additional control samples is in progress. We found no significant association of ATG5 polymorphism (rs17587319) with increased asthma risk (OR = 2.1778, 95% CI=0.4536- 10.4566, P = 0.3309). These results indicate that ATG5 (rs17587319 C/G) does not predispose individuals to asthma in the north Indian population.

Biography

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