中国P站

Abstract

Lymphatic filariasis is a neglected tropical disease, causing a major health hazard in the developing world. WHO has ranked the disease as one of the world鈥檚 leading causes of permanent and long-term disability. The currently available anti-filarial drugs are most effective against microfilaria. Therefore there is urgent need of drug that are macrofilaricidal. Nanoparticles have gained significance in medical fields due to their high surface-area-to-volume ratio. In this study, we synthesize AgNPs from a medicinally important plant Andrographis paniculata. The plant have been reported for their antimicrobial, cytotoxicity, anti-protozoan, antiinflammatory, anti-oxidant, and antiparasitic activities. This nanocomposite was characterized by UV-visible spectroscopy, FT-IR, XRD, SEM, and TEM. Nanocomposite anti-filarial activity was evaluated using motility and viability assay as well as by measuring ROS generation, antioxidant level, and apoptotic markers. The exposure of the nanocomposite to the worms caused a significant decrease in motility and viability leading to their death. Down-regulation of the antioxidant enzymes, as well as alteration in Ca2+ signaling, suggested the ER stress-induced mitochondrial-mediated apoptosis. The proteome analysis of treated parasites showed the marked alteration in the protein expression in comparison to the control. In conclusion, the nanocomposite synthesized using plant A. paniculata showed strong anti-filarial activity.

Biography

E-mail: yadav.smita7@gmail.com