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Microalgae offers a great potential to produce oils for the biodiesel industry but the cost and feasibility require further
investigation into finding fascinating species that have both a high lipid content and robustness in unusual growing
conditions. The Egyptian environment is characterized by sunny weather throughout the year and the availability of different
water sources are represented by the Mediterranean Sea and the Red Sea. In addition to this, there are many lakes and
canals which can offer great opportunities for growing microalgae for different purposes. This presentation explains the
characteristics of some microalgae species inhabiting the intertidal region of the Egyptian Red Sea shores between Quoseir
and Marsa Alamcities in Egypt and verifies the validation of different suitable conditions in Nile Red (NR) assay using green
alga Nannochloropsis salina to detect and quantify intracellular lipids. In addition, it shows the ability of some species to
grow under high temperatures (40 oC) and visualizing the formed lipid vesicles inside algal cells using the confocal LSM510
laser microscope. The results revealed that there was an accumulation of these lipid bodies which is the backbone of biodiesel
production as a response to temperature stress. Moreover, the molecular identification of the promised species was done.