ÖйúPÕ¾

Abstract

Ulcerative colitis (UC) is a type of inflammatory bowel disease, considered as an important disease of gastrointestinal tract having a huge impact on the health of the patient. The current study was performed to dissect out the site specific miRNA expression in the colon biopsy samples of UC patients from Northern India. Biopsy samples were collected from UC patients and healthy controls from Rectosigmoid area (RS) and Ascending Colon (AC). MicroRNA expression was compared between patients with RS and AC using a microarray platform. Differential expression was further validated by Real Time PCR analysis. Upon analysis of data generated on a microarray platform and qRT PCR revealed that the expression of six miRNAs hsamiR- 146b-5p, hsa-miR-335-3p, hsa-miR-342-3p, hsa-miR-644b-3p, hsa-miR-491-3p, hsa-miR-4732-3p were downregulated in patients where RS was involved as compared to AC. The expression of hsa-miR-141-3p was upregulated in patients where RS region was involved as compared to AC. Analysis of the registered UC patient�s database from the hospital revealed that the site of CRC was predomimnantly the rectosigmoid region of the colon in most of the cases. This is the first study to show the differential expression of miRNA involving different sites of colon in UC patients. Taking our data and previous reports into consideration, we propose that differential miRNA expression during UC perhaps contribute in the development of UC associated CRC at the rectosigmoid area.

Biography

Email: jpaul33@hotmail.com