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Abstract

We report results of the studies relating to the fabrication of nanostructured zirconia (ZrO2) based immunosensor for cardiac troponin I biomarker (myocardial infarction) detection. One step, low temperature hydrothermal process has been used for the synthesis of nanostructured ZrO2 (4.5 nm). These ZrO2 nanoparticles have been functionalized with 3-amino propyl triethoxy silane (APTES) and thereafter electrophoretically deposited (15 V, 3 minutes) on indium tin oxide coated glass electrode (ITO). EDC/NHS chemistry has been used for covalent immobilization of anti-troponin-I (anti-cTnI) onto APTES/ZrO2/ITO electrode. Structural, morphological and functional characterization of the synthesized nanoparticles and the fabricated immunoelectrode have been carried out via X-ray diffraction (XRD), transmission electron microscopy (TEM), atomic force microscopy (AFM), fourier transform infrared spectroscopy (FTIR) and cyclic voltammetry techniques. The results of electrochemical response studies of BSA/anti-cTnI/APTES/ZrO2/ITO immunoelectrode reveal that this smart platform can be used for the detection of troponin I biomarker with a wide linear detection range (0.01 to 100 ng mL-1), high sensitivity (3.7Ã?Â?10-4 mA mL ng-1 cm-2), remarkable lower detection limit (0.036 ng mL-1) and stability of upto 30 days. The obtained results have been validated through enzyme linked immunosorbent assay (ELISA).

Biography

Email: divyadubey09@gmail.com