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Abstract

L-asparaginase is an enzyme of clinical significance and highly applicable in the food industry with huge market demand. It is mainly used in the treatment of acute lymphoblastic leukemia. Till date, the major sources for commercial production of L-asparaginase are Escherichia coli and Erwinia carotovora bacteria. But, due to low yield and intracellular production, the problem arises in the extraction and purification of this enzyme. Side effects like thrombosis, pancreatitis, hepatotoxicity arises during course of treatment is also associated with it. To overcome these problems, industry is in constant search of better L-asparaginase producer strains with minimum toxicity. Rapid screening of L-asparaginase producing microorganisms is typically done on phenol red containing medium and identified by the change in color from yellow to pink and pink zone formation in the plate. The zone obtained is not very clear and sharp serves the basis of exploring other dyes for better contrasting zones and easy screening. In the present study different dyes like methyl red, bromocresol green, phenophthelin, bromocresol purple are investigated to detect the L-asparaginase producer strains with better resolution of zone formed compared to phenol red. These dyes could be used as an alternative and prove more accurate and sensitive to detect the presence of extracellular L-asparaginase produced.

Biography

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